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  • Q1:Write a 1500-word response (excluding references) to the question below. Use APA citation. The anterior cingulate cortex (ACC) is found near the front of the brain and wraps around the head of the corpus callosum. Seizures arising from the cingulate gyrus can be difficult to diagnose and treat due to their deep and medial location. To improve the outcome of the epilepsy surgery in cingulate epilepsy surgery, it is important that we understand cingulate cortex and its epilepsy. Describe common clinical seizure semiologies of the cingulate? Explain how this seizure type is classified? Discuss the role of non-invasive investigations in non-lesional cases. Include a discussion on the localisation of ictal EEG Explain why SEEG technique is favourable to explore the cingulate cortex compared to other invasive techniques. Compare and contrast subdural grid and SEEG technique? Describe challenges and pitfalls of diagnosis and treatment of medically refractory cingulate seizures.See Answer
  • Q2:Part I: Multiple Alignment A. Go to the EBI multiple sequence alignment tool page: (http://www.ebi.ac.uk/Tools/msa/) B. Click the "Launch Clustal Omega" link and at step 1 paste the CLUSTAL query from the Week 4 links page into the sequences in any supported format window. C. At step 2, click on "output format" and change it to Pearson/FASTA. Now click "submit" and wait for your job to finish. D. When your alignment job finishes, take a picture of your output and insert it here. E. Now click on the "Phylogenetic Tree" tab. This shows the tree used to construct the alignment. Take a picture of your tree and insert it here. F. You can create much nicer and more useful outputs using Mview. Click on the "Results Viewer" tab and then "View in MView" button. At Step 2 select input format: automatic. At step 3 on the MView submission page you can customize how your alignment is colored and formatted. Take a picture of your Mview output and insert it here. G. Return to the EBI multiple sequence alignment tool page: (http://www.ebi.ac.uk/Tools/msa) Repeat steps B-D, except click "Launch T-Coffee" for part B. H. Click on the "Phylogenetic Tree" tab. This shows the tree used to construct the T-Coffee alignment. Take a picture of your tree and insert it here. I. Now send your alignment to MView. At step 3 on the MView submission page be sure to use the same settings as for the Clustal Omega alignment. Take a picture of your Mview T- Coffee output and insert it here. J. Return to the EBI multiple sequence alignment tool page: (http://www.ebi.ac.uk/Tools/msa) Repeat steps B-D, except click "Launch MAFFT" for part B./nB. K. Click on the "Phylogenetic Tree" tab. This shows the tree used to construct the MAFFT alignment. Take a picture of your tree and insert it here. L. Now send your alignment to MView At step 3 on the MView submission page be sure to use the same settings as for the Clustal Omega alignment. Take a picture of your Mview MAFFT output and insert it here. M. Do you see any differences between the three alignments in the order in which the sequences are listed? N. Do you see any differences between the three alignments in the consensus? O. Do you see any other differences between the three alignments? P. Which alignment program would be most appropriate according to the table included in this week's lecture?/nQ. What other alignment methods might have been appropriate? R. What settings (if any) may have increased the quality of the alignment for ClustalOmega or MAFFT? Part II: Finding motifs A. Why is it useful to find motifs? B. Why might motifs be missed by global alignment programs? C. Why can motifs tell us about a group of proteins? D. Copy the "Clustal query" sequences, then go to the meme website http://meme- suite.org/tools/meme a. Select "classic mode" b. Under "Input the primary sequences" select "type in sequences" and then paste the CLUSTAL query from the Week 4 links page into the sequences under input primary sequences "10" for Maximum number of motifs to find then click the "start search" button. When your job finishes use the link at the top of the page to see your results. 1. How many motifs did it find? 2. Did each sequence have the same motifs in the same order?/nB. Now copy the sequence for gi|1084385 then analyze it with Motif Scan at prosite: http://myhits.isb-sib.ch/cgi-bin/motif scan (make sure you check all of the databases below the sequence window) 1. How many motifs did it find? 2. How many were significant (i.e. had a blue exclamation point)? 3. Were there any differences between the two sites? C. Now copy the sequence for gi|1084385 then analyze it with interproscan http://www.ebi.ac.uk/Tools/pfa/iprscan/ 1. What homologous families does this protein belong to? 2. What motifs did it identify? Were any not previously identified?See Answer
  • Q3:1. Enzymes: Biological Engineering to Support Bioreactors and Biosensors Enzymes in Weapons Production and Weapons Defense - • Enzymes in Weapons in Bioremediation - a report of at least 2 pages in length (1.5 spacing) with citations (pictures and diagrams are welcome and accepted (and improve your grade) but do not contribute to the 2 pages of report). Please include at least three examples with your general topic description. ReferencesSee Answer
  • Q4:2. Read chapters 5 through 10 and chapters 12 through 14 and other sources. To research the predominant bioengineering careers in the past, those which currently comprise the market and what is forecast for the future. What are the major career options, and where is this going in the future? ReferencesSee Answer
  • Q5:3. Use all resources available to find and describe the biomedical engineering code of ethics, and compare it to the Hippocratic Oath. (Minimum length - approx. 1 page) ReferencesSee Answer
  • Q6:- *S5: Discuss appropriate and informed career management choices and entrepreneurial issues* - Consider including a section on the practical applications of studying flaviviruses and pseudotyped viruses in terms of career opportunities and entrepreneurship.See Answer
  • Q7:1st submission Student needs 2 Jobs and 2 Business (companies names) Based on instructions of previous work topic Poster Title New strategies to produce pseudotyped viruses to study entry of Flaviviruses Synopsis A pseudotyped virus (PV) is a viral particle harbouring the core structure of one virus and the envelope (Env), the viral protein which interacts with the cellular receptor mediating entry, of a heterologous virus: these PV have been largely employed in gene therapy, serology and virus-host interaction studies. The use of PV is particularly important to study viruses classified as hazard group 3 and 4, as they can be handled at a lower containment level (BSL1/2) and do not require enhanced security, such examples are Ebola and other filoviruses, Lassa fever, Coronaviruses. However, those viruses which assemble within the intracellular membrane systems are particularly difficult to pseudotype, as the incorporation of their Env on the surface of a PV is compromised by the endoplasmic reticulum (ER) retention signal present on their Env. These include viruses which have caused or have the potential to cause an outbreak such as flaviviruses, e.g. Zika virus, Dengue virus and Japanese Encephalitis virus (IEV). Please note: The poster title has been set and the title cannot be changed.See Answer
  • Q8:2nd submission It should be exactly like this How does this relate to business and entrepreneurship plus career management? Which businesses have seen an opportunity so far? smartCells go Cord blood and adult stem cell storage company based in the UK offering package options for approximately 2000 plus c100 per year storage fee. BlueRock Therapeutics A joint venture between Bayer and Versant Ventures who have both invested a combined $225 million dollars. The company mainly focuses on technologies around cardiovascular, neurological and autoimmune applications currently. What careers and jobs are available in this sector? Bioinformatics Specialist to T Novo Foundatier for Stem and Protein Research (CP Location tom) Public Engagement Officer Location London, United Kingdom Organisation Centre for Stem Cells and Regenerative Medicine (CSCRM) Laboratory Technician Location: Copenhagen, Denmark Organisation: DanStem What is the industry worth? The global stem cell market size was estimated to be at 56.87 billion in 2016. Transparency Market Research estimate that the global market will be worth $270.5 billion by 2025 research for the job and business/nIt's a group presentation my section is employability I will talk about the jobs and business and how it's related to the job.I will be talking about how we apply this knowledge into real world ... And then I will talk about the jobs and the difference between them and I will say why would I go for this or that job... and then I will talk about the business the example of the business... Career Management Chofces b Job 2 Why لاد Job ل Why Add referebces abd if there any photo of the company logo IdeaSee Answer
  • Q9:Fundamentals of Scientific Inquiry We have discussed science throughout history this term, paying particular attention to the paradigm shifts that occurred as scientists reorganized their data to fit with a model that had greater explanatory power. Research a paradigm shift within a particular field of science that we have not covered in class. Choose a particular scientific field (medicine, geology, biology, microbiology, genetics, physics, anatomy, ecology, astronomy, neuroscience, engineering, etc.) Choose a time in which this field was undergoing a great change in thinking or methodology. Argue for whether or not this time in history was a true paradigm shift, given what you know of Thomas Kuhn's definition. In order to do this, you should examine the differences and similarities between the previous era in this scientific field and the one that came after, with reference to the models and methods used and discoveries made.See Answer
  • Q10: Which of the following is a limitation of surface EMG measurements? See Answer
  • Q11: What trend best matches what you observed for the changes in EMG magnitude as weight is increased? See Answer
  • Q12: Make a graph of column F in the isometric weights spreadsheet (50 lb column). What part of the typical processing of EMG does the signal in the F column look like? See Answer
  • Q13: What was the trend of mean amplitude over time in the EMG fatigue spreadsheet? See Answer
  • Q14: Which of the following are likely reasons that there is not a perfect fit to the trendline observed in the isometric weights spreadsheet? See Answer
  • Q15: What was the trend of median frequency over time in the EMG fatigue spreadsheet? See Answer
  • Q16: Why would the amplitude of EMG show an opposite trend to median frequency in the EMG fatigue spreadsheet? See Answer
  • Q17:Submit your solutions on Gradescope. 1. [Total: 16 pts] The figure below shows a schematic control block diagram of the control of respiration, with the respiratory controller representing the respiratory chemoreflexes, brain respiratory neural centers and the respiratory muscles, and the lungs representing the CO₂ exchange in the lungs. In amyotrophic lateral sclerosis (ALS, also known as Lou Gehrig's disease), motor neuron degeneration results in weakness and eventual paralysis of the respiratory muscles. Note that the regulation of ventilation model here is slightly different (more simplified) from the one we discussed in class. V/₁ Respiratory controller Lungs Pacoz a) [2 pts] Suppose that, in a particular ALS patient, the (steady-state) respiratory controller equation (which characterizes the chemoreflex response) is given by the following equation: V₁ = Paco2 - 37 Where V represents the alveolar ventilation (in L/min), and Pacoz is the partial pressure for CO₂ in the arterial blood (in units of mmHg). Draw (as accurately as possible on the provided graph) the line representing the steady-state controller response to Paco2- Note that we are neglecting the effects of O₂ here. Label this line as "(a)". b) [2 pts] Assume that gas exchange in this patient is normal and can be characterized by the following plant equation: Paco2 = 200/V Draw (as accurately as possible on the provided graph) the relationship between Pacoz and V. Label the curve as "(b)"./nVdotA (L/min) 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0.0 40 41 (mm Hg) c) [2 pts] Estimate the steady-state operating values of Pacoz and V₁ in this patient. d) [4 pts] In a normal healthy person, the controller response is given by V₁ = 2(Paco2-37). Draw this controller response on the graph below. Estimate the steady-state operating values of Paco2 and V in the normal subjects. Label the curve as "(d)". 35 36 37 38 39 P aCO2 42 43 44 45 e) [4 pts] A pressure support ventilator can be used to provide assistance to the ALS patient - such a ventilator would produce an increase of x L/min on top of the subject's own natural ventilatory output, independent of the Pacoz level. However, the ventilator has to be triggered by a minimal inspiratory effort by the patient himself. If the patient does not generate any effort, the ventilator does not provide any ventilatory assistance. Draw on the graph below the total controller response (patient+ ventilator) required to restore the steady-state V and Pacoz values to the levels seen in the normal subject. Label the total controller response as "(e)". f) [2 pts] What is the value of x (L/min)?See Answer
  • Q18:2. [15 pts] Assume the metabolic hyperbola for CO₂ given by the following equation: 863 - Vcoz V₁ PACO2 Pico2+ where the normal steady-state CO₂ production is 235 mL/min and the inspired CO₂ concentration (or volumetric fraction) is 0. Also, assume a dead-space ventilation rate of 1 L/min. Also, suppose the steady-state ventilatory response to CO₂ is given by the following equation: 32 Vc = Drive external +(1.46 + (Paco2-37) and Vc 20 Pa02-38.6) Note that the above equation is slightly modified from what we derived in class in that a constant term, Drive external is added to the equation. This drive represents an additional source of control that affects ventilatory response e.g., sleep-wake state, alertness, etc. At normal state (resting and awake), let Drive external = 0. a) [5 pts] Use MATLAB to plot the metabolic hyperbola for CO₂ and the ventilatory response to CO₂ under normal condition. Show both graphs on the same figure (see hold function). Label both axes with appropriate units. Also indicate which graph represents the me the metabolic hyperbola for CO₂ and which graph represents the ventilatory response to CO₂ (see legend function). The plot should show the range of ventilation from 0-20 L/min and the range of PACO₂ from 30-60 mmHg (see xlim and ylim functions). Determine the normal steady-state values of ventilation and Pacoz from the plot (e.g., click on the plot where you want to obtain the x-y coordinates). Note: For the metabolic hyperbola for CO₂, please plot V against PACO₂ i.e., you would take the dead-space ventilation into consideration. b) [4 pts] The onset of sleep causes the external drive to breathe to drop by -5 L/min. State which parameter changes and to what value. Show the graphs representing this situation and report the steady-state values of ventilation and Pacoz. c) [4 pts] Follows from b), how would inhalation of a gas mixture containing 3% CO₂ in air (i.e., volumetric fraction of 0.03) during sleep affect the steady-state values of ventilation and Pacoz? State which parameter changes and to what value. Show the graphs representing this situation and report the steady-state values of ventilation and Pacoz. You may assume that the subject is at sea level. d) [2pt] Copy and paste the MATLAB code you used in this question (all parts). You may take a picture of your MATLAB script, but please make sure that the code is clearly readable from the image.See Answer
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