CY6065 CB Feb 2024 Water Maze Comprehension Datasheet ⚫ For your answers, do not do a full report; simply answer the questions exactly as numbered below (a) to (d). Work that is not clear or not presented in a professional, logical, concise way will lose marks. Marks will be deducted for: - pages of irrelevant statistical data; - untidy / poorly assembled work; - poor explanations; - inappropriate statistical comparisons. The usual rules regarding plagiarism apply - please don't! Check with me (CB) if you are not sure. Work must be submitted on-line as a single file via "on-line submission" in Weblearn./nQuestions 1. Does scopolamine produce a cognitive defect as demonstrated by significant changes in the performance of animals in the water maze? 2. If there is an effect, is this changed by the concurrent administration of the anticholinesterases neostigmine or physostigmine? a) From the Excel spreadsheet provided, calculate (i) the t-test p-values for each day for each treatment group compared with its appropriate control(s) using Excel's two sample t- test assuming equal variances. (ii) Calculate the means of your data sets + SEMS (standard errors of the mean), and present these, along with the p-values, in three tables (scopolamine vs control ("vehicle"); scopolamine vs scopolamine plus neostigmine; scopolamine vs scopolamine plus physostigmine). 20 marks b) Plot the means of your data onto a total of two line graphs. You may use hand-drawn graphs, or you can use appropriate software. Graphs should be of path length vs time (i.e. day number). One graph should show scopolamine data vs vehicle data (i.e two sets of data) over 4 days. The other graph should show the effects of concurrent administration of each anticholinesterase with scopolamine, compared to scopolamine alone (three sets of data). For each data point add SEM error bars and asterisks denoting statistical significance. Please do not submit photographs of graphs, use scanned images. 30 marks c) Give a professionally-written summary of your findings by addressing questions (1) and (2) above, and justify your answers using your statistical data (p-values) judiciously (max. 400 words total). Your answer should include a comment on the probable pharmacological mechanisms involved (include references where appropriate). d) Referencing 45 marks 5 marks/nCY6065 The Effects Of Various Treatments On The Rats' Path Length P-value 0.847492442 0.007494998 0.0000001095346527 Vehicle mean: +SEMS Scopolamine mean ±SEMS Day one 1182.83333 ± 42.173780618 | 1173 ± 26.52545947 Day two 732.5±1590964068 Day three 339.8333± 69163994 Day four 100.6666 ± 2.2311 822.1667 ± 21.6278 570.5±12.78997003 366.8333± 8.9978 0 question A Table one: vehicle and scopolamine treated rats over four days. Neostigmine plus Scopolamine mean ±SEMS P-value Scopolamine mean ± SEMS Day one 1183.3333 ± 28.44371 1173 ± 26.5254 0.7576804982 Day two 824.8333 ± 29.9749 822.1667 ± 21.6277 0.9439095198 Day three 571.3333 ± 8.3093 570.5 ± 12.7899 0.9575037175 Day four 354.5±4.5074 366.8333± 8.9978 0.2484558611 Table two: neostigmine pulse scopolamine and scopolamine treated rats over four days. Scopolamine plus Scopolamine mean ± SEMS P-value physostigmine mean ± SEMS Day one 1088.333333 ± 33.911322227 | 1173 ± 26.52545947 0.07758663323 Day two 758.5 ± 16.96810734 Day three 311.5 ± 8.872241355 822.166666666667 ± 21.62778563 570.5±12.78997003 0.04306311033 0.0000000128595157 Day four 101.666666 ± 3.508719298 366.833333333333 ± 8.997839247 0 Table three: scopolamine pulse physostigmine and scopolamine treated rates over four days.

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