Data handling 2. Interpret the results presented in the provided figures to answer the questions below. The word count of the data handling is no more than 750 words. The data

presented were generated in experiments where atomic force spectroscopy is applied to measure plasma membrane tensions in naïve versus primed pluripotent stem cells. (a) First explain the difference between naïve and primed ESC. [10 marks] (b) Assess the key data from the publication below, explain the relevance of the observations and describe broadly how statistics are used to conclude an outcome. CAEzrin is a constitutively active form of the cytoskeletal protein Ezrin and iMC is a synthetic analogue of constitutively active ezrin. [20 marks]/nGeoMean Nanog (normalized (c) mESC expressing CAEzrin or iMC also express higher levels of the transcription factor Nanog. Describe the data below and its relevance in terms of ESC pluripotent state. [10 marks] 20- 1.0 0.5- 0.0- 0.0076 0.04 đơn th 48h +CAEZ +MC- Light Green=Primed mESC Orange CAEzrin-mESC Purple iMC-mESC = Normalized geometric mean intensities of Nanog immunofluorescence levels for CAEZ and ind-IMC mESCs plated for 48 h in N2B27 medium or N2B27 medium. Error bars, SEM; p values, Welch's t test.