A260=1.446
A280 = 0.784
Based on the data above,
A) Briefly describe the protocol for DNA isolation.
B) Comment on the purity of the DNA sample obtained. Provide appropriate calculations to back up your comment.
C) Assuming a pure solution of double-stranded DNA has a concentration of 50 µg/ ml and A260 of 1.0, calculate the concentration of DNA in the solution obtained by the scientist and give the total DNA yield.
D) How can the scientist assess DNA breakage in the sample obtained?