Question

1. The rate at which a given enzyme can catalyse a reaction (v) can be dependent on the substrate concentration (s) according to the relationship v=\frac{s}{m+s c} where m and

c are constants. In an experiment measuring reaction products over time, the rate v was recorded as concentration s was increased, and the results recorded in the table: (in umol dm-3 min-1) 0.05 0.100.20 0.290.38 0.480.65 0.740.83 0.91 (in umol dm-3)10203040 50708090 100 (ii) Predict the rate of reaction when s = 155 umol.dm-3. How reliable do you think this value is? Give reasons for your answer. (i) Verify graphically (i.e. plot using graph paper) that these data follow the relationship above,and hence determine c and m. NOTE that to find m you should take the line of best fit to the data points NOT just use two of the data points. What are the units of c and m?

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