Question

3. The recognition cutting sites of 2 different enzymes (enzymes A: blue star; enzyme B:

red triangle) are shown in the map below. In your answer include:

a) A prediction of the size (in bp) of the resulting fragments after digestion of the amplicon with

the two enzymes mixed together (Mix), when each enzyme is used separately (A and B) and

when no enzyme (NO) is used.

b) A drawing of these fragments onto the graph below to represent a gel electrophoresis.

You can either recreate the picture e.g. in PowerPoint/paint/others and then paste this in your

research paper or print this, draw on it, and take a picture of it and then upload this on the

research paper. Doesn't need to be perfect but the bands size difference MUST be clear.

Fig: 1