Search for question
Question

(a)Describe the methods used to study protein structure (give a list of methods mentioning the achievable resolution and sample requirements for each method). (b)You are given 100mg of a stable, soluble and pure 500 kDa protein. Which low- and high-resolution techniques would you use to obtain structural information of the protein? Which methods would you not use and why?Discuss the advantages and disadvantages of each method. (c)Why do some proteins crystallize easily while others do not? Focus on the properties of different proteins. (d)Discuss the differences between different amino acids (discuss amino acidgroups and list amino acids belonging to each group). Draw the structures ofarginine and alanine and compare these 2 amino acids.

Fig: 1

Fig: 2

Fig: 3

Fig: 4