BMOL20070 Assignment 3 (Practicals 4-8-9) You must answer all questions typed and submit as a .pdf on Brightspace. The deadline for Assignment 3 is April. The Assignment component for BMOL20070 is

woru 35%0. Al 5 assignments are equally weighted. Remember, late submission will incur in loss of marks. Practical 4: Rat Ileum Question 1. Carbachol causes contractions the isolated rat ileum to contract. The responses obtained to increasing concentrations of carbachol are tabulated below. Graph the data as log concentration versus % maximum response, using Excel or another spreadsheet program. Use the graph to estimate the EC50 value for carbachol and indicate its position on the graph. Include the graph in your submitted file. Conc (M) 1.00E-09 3.00E-09 1.00E-08 3.00E-08 1.00E-07 3.00E-07 1.00E-06 3.00E-06 Exp 1 (% max response) 0 10 45 57 74 95 100 100 Exp 2 (% max response) 0 9 35 69 81 85 100 98 Exp 3 (% max response) 0 20 48 72 91 96 99 99 Question 2. Briefly outline an experiment that would determine the relative potencies of two agonists acting at different receptors e.g. carbachol and serotonin.