Disinfection: Chlorination of Wastewater Effluent
Note: the original version of this laboratory experiment was written by the Association of Environmental
Engineering Professors (Unit Operations and Unit Processes Laboratory Manual, 4th edition, 1998). In
Spring 2005, Shamin Begum (graduate assistant) and Dr. Jacimaria Batista modified the laboratory to fit the
coliform bacteria equipment available at UNLV.
Learning objectives:
1. Students should be able to know what coliform bacteria are and why they were chosen as a
measure of microbiological quality of waters.
2. Students should be able to explain the meaning of "concentration vs. time" as applied to
disinfection of waters.
3. Students should be able to use an MPN table to interpret experimental data.
4. Students should be able to experimentally determine the inactivation rate constant and the
coefficient of dilution.