Materials used: Hydrogen peroxide (3% H₂O2 diluted in PBS) - 1x PBS (0.15 M) – Blocking solution (1.5% Normal Horse Serum diluted in PBS) Primary antibody (mouse anti-PCNA, 1:300 diluted

in blocking solution) -Secondary antibody (Biotinylated horse anti-mouse IgG, 1:200 diluted in blocking solution) ABC reagent AEC substrate DESCRIBE EACH IMAGE INCLUDING KEY POINTS: Each of your images needs to include: 1) Magnification - each image lens x40 2) Labelling examples of all key structural components including, but NOT restricted to, different types of cells and nuclei present on the image, with precisely positioned label lines and scientifically correct labels 3) Labelling examples reflecting your experimental design, including immunostaining, non- specific staining, and counterstain, if present in the image, with precisely positioned label lines and scientifically correct labels Each image legend needs to include: 1) Brief indication of tissue treatment or the experiment design 2) Description of the key information about immunostaining present in the image, reflecting on your experimental design, with clarity and simplicity 3) Description of cell and tissue morphology or morphological changes Liver tissue, normal Cancer liver tissue, treated with primary and secondary antibody, no treated with H2O2 Cancer liver tissue, treated with primary antibody, H2O2, no treated with secondary antibody Cancer liver tissue, treated with H2O2, secondary antibody, no treated with primary antibody Cancer liver tissue treated with H2O2, Primary and secondary antibody