PART A: Multiple Choice Questions (5 marks) 1. EcoR1 is a tool used in the ‘cutting’ manipulation of DNA. EcoR1 is a 2. When carrying out polymerase chain reactions (PCR), DNA engineers do not use human enzymes. Instead, they use a bacterial enzyme derived from a hot spring-dwellingmicroorganism. Why is this? 3. When carrying out DNA manipulation, a vector is used to 4. Bt cotton make up much of the cotton planted in Australia. They are genetically modified and contain a gene from bacterium Bacillus thuringiensis, which allows them to produce a protein that is toxic to insects. A social implication of Bt corn could be: 5. Four different plasmids, were each incubated with the restriction enzyme Eag 1. Each of the plasmids were then sorted on an electrophoresis gel. The results are shown below.

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