Standard constants: Take quiz Exit = 1 tonne 1,000 kg Atomic masses: C = 12, H = 1, O=16, N = 14, S 32 T(°c) =T(K) - 273 R = universal gas constant = 8.314 J mol-1 K-1 = == 1.987 cal mol 1 K-1 Question 1 2 pts The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH 7.0 and 20 °C to produce Glutamic acid for the manufacture of Mono Sodium Glutamate, a flavour compound for the food industry. The time course data for a typical batch culture of this bacterium in a fermenter vessel are given below: Take quiz typical batch culture of this bacterium in at fermenter vessel are given below: Cell Time Glucose Glutamic acic (h) concentration concentration concentration (g 1-1) (g 1-1) (g 1-1) 1.222 100 0 5 1.492 98.0 2.5 10 1.649 97.0 7.5 15 2.719 90.4 20.0 20 4.482 76.9 34.0 25 7.390 48.1 43.0 30 12.183 20.6 47.5 35 20.086 3.4 48.0 40 21.250 1.1 48.3 45 21.260 0.1 48.5 Exit Take quiz Exit 35 20.086 3.4 48.0 40 21.250 1.1 48.3 45 21.260 0.1 48.5 Based on the above data, determine the maximum specific growth rate of Corynebacterium glutamicum. O 0.1 min 1 O 0.063 h-1 0.0034 min 1 O1s 1 O 0.08 g 1-1 s-1 0.003 h-1 O 0.1 h-1 O 0.74 h-1 21.2 s 1 none of the above Question 2 Take quiz Exit ...continuation from question above... 2 pts The fermenter vessel is to be harvested for the glutamic acid in the liquid phase, once the carbon substrate (glucose) is completely consumed. How many production scale fermenters, each of 150 m³ operating liquid volume, would be needed to make 10,000 tonnes per year of Glutamic acid? Each batch. vessel is operated for the harvest time of complete carbon substrate utilisation (45 hours), and takes an additional 3 hours to load with liquid medium and inoculate with bacterial culture, and 2 hours to drain and sterilise after the aerobic batch fermentation. Take a process year to be 325 days. (Write in the box below the number of fermenter vessels needed. Do not enter any text, only a number)