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5. Which experimental treatment (Easy or Difficult) has choice-selections that are most risky for the survival of Physarum and why? How do we expect results to differ between the easy and difficult treatments? BIOL300 Laboratory Name: Quantitative Analysis Instructions: Perform the following statistical tests and transcribe the results in the tables below. All tests should be two-tailed using a = 0.05. Tests can be completed at: http://www.socscistatistics.com/tests/Default.aspx Easy Choice Difficult Choice Lab 3.1 Page 1 of 3 z-tests 1a. Perform a z-test (for two population proportions) to test whether there is a significant difference between the proportion of starved and well-fed Physarum that made accurate choices (picked 10%) in the Easy Choice Experiment. 1b. Repeat the same test using the data for the Difficult Choice Experiment. t-tests 2a. Perform a t-test (independent means) to test whether there is a significant difference between the mean time-to-decision of starved and well-fed Physarum in the Easy Choice Experiment. 2b. Repeat the same test using the data Difficult Choice Experiment. Results Table 1. Results for a z-test on the difference in accuracy between the proportion of starved and well-fed Physarum in the Easy and Difficult Choice Experiments. Easy Choice Difficult Choice Z-score Lab Section: t-score p-value Table 2. Results for a t-test on the difference in time-to-decision between the starved and well-fed Physarum in the Easy and Difficult Choice Experiments. p-value Significant difference? Significant difference?


3). Some characters has/have derived states that are shared by two or more taxa in the matrix. This type of character is called a synapomorphy (shared derived) and is the basis for building phylogenies. For each synapomorphic character, list the taxa that share it.


4). Reconstruct the phylogeny that most simply and accurately accounts for the distribution of synapomorphies among ingroup species. Given matrices as small and simple as the above example, one can "build" the tree from the bottom up in a series of sketches, adding clades or branches representing groups with the fewest synapomorphies near the base of the tree and those with the most at the tips. Using the unlabeled phylogeny as a starting point, draw the stepwise construction of a fully resolved phylogeny to account for all of the synapomorphies, and include the synapomorphies that identify each clade (the first one, character 2, is included to get you started; this is shared by all members of the ingroup). OG Character 2 (leaf edge): 0-1


5). Map the one autapomorphic character onto the branch leading to the single species that has a derived character state. These types of characters provide no grouping information.


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