1. Consider the following chemical reaction catalyzed by the enzyme phosphoglycerate mutase: Phosphoglycerate = 2- phosphoglycerate When a mixture of 3-phosphoglycerate (3-PG) and 2-phosphoglycerate (2-PG) is incubated at 1 atm,

pH 7, 25°C with phosphoglycerate mutase until equilibrium is reached, the "nal mixture contains seven times (7X) more 2-PG than 3-PG. a) [1 mark] Calculate AGº of this chemical reaction. Show your work! b) [2 marks] In a cell at 37ºC, the concentration of [3-phosphoglycerate] is 3.6 x 10°³ M and [2-phosphoglycerate] is 5.2 x 10-4 M. Calculate the AG’ for this reaction and explain if the reaction would spontaneously proceed in the forward direction. Show your work! You will not receive full marks unless you show your work!! c) [2 marks] Suppose that the enzyme phosphoglycerate mutase were placed into a solution containing only0.25 mM 3-phosphoglycerate at 25°C, 1 atm, pH 7. The enzyme was left in the solution until the reactionreached chemical equilibrium. What is the "nal concentration of [3-phosphoglycerate]?