2. In the TCA cycle, an enzyme called fumarase catalyzes the following reaction: The AGo' of this reaction is -0.907 kcal/mol. Answer the questions below. HINT: Remember you do not

need toinclude water in the mass action expression. a) (1 mark) If you know the AGo' < 0 for the reaction above, can you say with confidence that this reactionwill proceed spontaneously to the right in a biological cell? Explain why or why not. b) (1 mark) Calculate the K'eq for the reaction catalyzed by fumarase under std. conditions. c) (2 marks) If the concentration of fumarate inside of a cell is held at 3.26 µM, how many grams of malatecan exist inside of a cell before the reaction will cease because it is no longer thermodynamically d) (2 marks) The equilibrium constant (K'eq) changes with temperature. From your calculations in part c), what is the equilibrium constant at 37.5oC?