21.) Below is a basic Lineweaver-Burk plot of a simple enzyme system w/ and without inhibitors at an enzyme concentration of 50 uM. Answer parts A-C. A.) Determine the Km, Vmax and kcat of the uninhibited enzyme (16 pts). Indicate whether each inhibitor displays competitive, uncompetitive or mixed inhibition. .) Determine the apparent VmaxªPP & KmªPP values for the enzyme in the presence of Inhibitor 1. ) If Inhibitor 1 is at a concentration of 40 uM, determine the Kı value for the inhibitor. ( Determine the appropriate K₁ and/or Kı' values for inhibitors 1 & 3 at concentrations of 40uM.

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21 below is a basic lineweaver burk plot of a simple enzyme system w a

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21.) Below is a basic Lineweaver-Burk plot of a simple enzyme system w/ and without inhibitors at an enzyme concentration of 50 uM. Answer parts A-C. A.) Determine the Km, Vmax and kcat of the uninhibited enzyme (16 pts). Indicate whether each inhibitor displays competitive, uncompetitive or mixed inhibition. .) Det