3. You create a fusion protein that adds a Green Fluorescent protein domain to thesoluble cargo protein in the diagram above. Where does the cargo proteinaccumulate if :a. Rab is mutated and does not function?b. The transmembrane cargo protein is missing?c. The coat protein is missing?d. The v-snare is mutated and does not function?e.The t-snare is mutated and does not function?f. The pH at the final destination is the same as the pH of the transportvesicle?