● CY6065 Water Maze Comprehension Datasheet For your answers, do not do a full report; simply answer the questions exactly as numbered below (a) to (d). CB Feb 2024 Work that is not clear or not presented in a professional, logical, concise way will lose marks. Marks will be deducted for: - pages of irrelevant statistical data; - untidy / poorly assembled work; - poor explanations; - inappropriate statistical comparisons. The usual rules regarding plagiarism apply - please don't! Check with me (CB) if you are not sure. Work must be submitted on-line as a single file via "on-line submission" in Weblearn. Questions 1. Does scopolamine produce a cognitive defect as demonstrated by significant changes in the performance of animals in the water maze? 2. If there is an effect, is this changed by the concurrent administration of the anticholinesterases neostigmine or physostigmine? a) From the Excel spreadsheet provided, calculate (i) the t-test p-values for each day for each treatment group compared with its appropriate control(s) using Excel's two sample t- test assuming equal variances. (ii) Calculate the means of your data sets + SEMS (standard errors of the mean), and present these, along with the p-values, in three tables (scopolamine vs control (“vehicle”); scopolamine vs scopolamine plus neostigmine; scopolamine vs scopolamine plus physostigmine). 20 marks b) Plot the means of your data onto a total of two line graphs. You may use hand-drawn graphs, or you can use appropriate software. Graphs should be of path length vs time (i.e. day number). One graph should show scopolamine data vs vehicle data (i.e two sets of data) over 4 days. The other graph should show the effects of concurrent administration of each anticholinesterase with scopolamine, compared to scopolamine alone (three sets of data). For each data point add SEM error bars and asterisks denoting statistical significance. Please do not submit photographs of graphs, use scanned images. d) Referencing 30 marks c) Give a professionally-written summary of your findings by addressing questions (1) and (2) above, and justify your answers using your statistical data (p-values) judiciously (max. 400 words total). Your answer should include a comment on the probable pharmacological mechanisms involved (include references where appropriate). 45 marks 5 marks