Question

Ex. 16: Identification of unknown bacteria How are bacteria identified in the lab? Huge machines run hundreds of the same biochemical tests we do in class to identify the kind of bacteria in the sample DNA of unknown pure cultures sequenced Fluorescently labeled ddNTPs T A ddTTP ddATP ddGTP ddCTP T A G DNA template Separate with a gel G A CTG A A G C A C T GAA C T G A A G C T T T G A A G C T G A A G C T A A G CT A G C T G C T Use a sequencing machine GACTGAAGCT Ex. 16: Unknown bacteria Take all you have learned up to this point to identify an unknown sample Each person will get an unknown! This is an individual activity Use skills such as isolation streaking, gram staining, identifying colony and cell morphology Follow the keys in the exercise to build evidence on what your unknown is Summary of results due at the end of the term Possible Bacteria Escherichia coli Citrobacter freundii Bacillus subtilis Bacillus megaterium Bacillus cereus Klebsiella aerogenes Proteus vulgaris Serratia marcescens Day 1 Get your unknown sample-keep track of culture # and any observations throughout the process in table 16.1 Do an isolation streak with your unknown. Streak at least 2 TSA plates Gram stain unknown. Is it a pure or mixed culture? Are cells of similar morphology? Begin doing metabolic tests as you see fit. Day 2 and onward... Observe isolation streaks from last period. Continue metabolic tests. Important to write down interpretations throughout the entire process in table 16.1. Confirm your results as you go./n/n

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