High-performance liquid chromatography (HPLC) also known as high-pressure liquid chromatography is a laboratory technique used to separate the components in a mixture, to identify each component, and to quantify each

component. It consists of a horizontal packed column with porous silica beads in which components are separated due to their different interactions with the silica beads. Typical specifications for the columns are:Problem 3. Column diameter: D = 4.6 mm Column length: L=250 mm Void volume: ɛ= 0.59 The recommended system flow rate is 1 mL/min, which results in a pressure drop of -AP =500 psi. Assuming the fluid is water and the flow is laminar, perform the following (38 points total): State five assumptions to solve this problem. ( b. Write and simplify (based on the assumptions) the basic equation(s) necessary to solve for particle diameter. (6 points) c. What is the diameter of the packed particles (Dp) in µm? d. Calculate the number of particles in the packed column e. What is the Reynolds number for 1 mL/min? Is the assumption of laminar flow valid? f. What is the maximum pressure (in psi) if the maximum flow rate is 10 mL/min?