Km Vmax calculation exercise Kindly solve the following question via Excel sheet and submit your trial: - Individual submission Refer to topic 4.Enzymes to solve the question. The initial rate of conversion of fumarate to L-malate using the enzyme fumarase was recorded at different fumarate concentrations. Inhibitors A, B and C were added. By using the data below, answer the following: 1.What is the Km and Vmax of the reaction without inhibitors? 2.What type of inhibitors were they? Fumarate [S] Rate of product formation With A (MM) (mmol l-1 min-1) (mmol l-1 min-1 2 2.5 2 3.3 3.1 2.7 5 3.6 3.1 10 4.2 3.9 2.What type of inhibitors were they? Fumarate [S] (mM) Rate of product formation With A With B With C (mmol l-1 min-1) (mmol 1-1 min-1) (mmol l-1 min-1) (mmol I-1 min-1) 2 2.5 2 2 1.4 3.3 3.1 2.7 2.35 1.7 5 3.6 3.1 2.6 2 10 4.2 3.9 3.1 2.35