Ⓒ Macmillan Learning

Determine the response factor (F).

F =

After establishing the response factor of the instrument, the researcher collected 13.98 g of spinach, homogenized the sample,

and extracted the DDT using an established method, producing a 3.19 mL solution containing an unknown amount of extracted

DDT. The researcher then prepared a sample for analysis that contained 0.500 mL of the unknown DDT solution and 2.00 mL

of 10.91 mg/L chloroform, which was diluted to a final volume of 25.00 mL.. The sample was analyzed using GCMS, producing

peak areas of 6393 and 14403 for the DDT and chloroform, respectively.

Calculate the amount of DDT in the spinach sample. Express the final answer as milligrams of DDT per gram of spinach.

amount of DDT:

mg DDT /g spinach