Observation of catalase in different Temperatures: Temperature Foam height (cm) 0.8 cm 0°C Room Temperature 37°C 50°C 70°C 1.2 cm 1.8 cm 1.5 cm 0.0 cm Reason Lower foam height due

to reduced enzyme activity at low temperatures. Increased foam height as enzyme activity improves with higher temperatures. Maximum foam height, the optimum temperature for catalase activity. Decreased foam height duel to enzyme denaturation at high temperatures. No foam produced due to extensive enzyme denaturation, rendering. catalase inactive. Question: 1) Based on the data obtained, plot a graph with the height-readings obtained (Temperature on X-axis versus height in cm on Y-axis). 10. From this graph determine the optimum temperature and also the denaturation temperature for the enzyme. Note on drawing graphs: Most of the graphs, which you will draw in biology, will have a vertical and horizontal axis: • Horizontal Axis (x-axis). The set conditions (independent variable) of the experiment is always placed on the horizontal axis. These include known criteria like pH or temperature, or some other condition. Label the axis with the name and units (e.g. temperature (°C)) and values. • Vertical Axis (y-axis). The results recorded (under the set conditions) are placed on the Vertical Axis. Prior to doing the experiment, these readings are unknown. Again, label the axis with the name (e.g. time (seconds)), units and values./nEffect of pH on catalase enzyme activity: Theoretical pH Vol. of NaH2PO4 (ml) 5.6 6.2 7.0 8.05 9.05 Question: 0.5 2.0 6.0 9.5 10.0 Vol. of KH2PO4 (ml) 9.5 8.0 4.0 0.5 0 Actual pH (verified with pH meter) 5.49 7.03 7.83 8.83 9.73 1) Identify the optimum pH? 2) Plot a graph of your results, pH against foam height. Foam level (cm) 1.55 3.3 2.9 2.7 2.3