Oxidation of NADH and FADH2 in the electron transport system differ in that:
Only NADH involves transfer of electrons from Complex II to Cytochrome C.
Only NADH involves transfer of electrons from Complex I to Coenzyme Q.
Only NADH results in pumping of H* into the inter membrane space.
Only NADH is oxidized by giving its electrons to succinate.
The Delta Plus variant of SARS-CoV-2 has been found to have an amino acid substitution in the spike protein known as K417N. The spike protein is considered to be how the viral particle attaches to the host cell for infection. Based on your knowledge of amino acid side chain chemistry, speculate how this amino acid substitution (i.e. N replaces K at position 417) could lead to an altered potentially improved interaction with the host protein receptor. Your answer should include the following:
a) Report the optimum pH of lactase:
b) How does this pH compare to the pH of the environment in which lactase ismost active?
c) Why is there no lactase activity at pH 2 and 10?
a) Which of the compounds tested, inhibit the activity of lactase?
Classify the inhibitor(s) as likely to be competitive, allosteric, irreversible or denaturing and explain your reasoning.
b) Which of the compounds tested, activate the activity of lactase?
Classify the activator(s) as likely to be competitive, allosteric, irreversible or denaturing and explain your reasoning.
Table 1 - Assessing the Specificity of Lactase
a) Which of the disaccharides tested is/are recognized and hydrolyzed by lactase?
b) What does this data tell us about the specificity of lactase?
c) A student added lactase to galactose B(1>4) galactose. Would you expectlactase to hydrolyze this disaccharide? Would you expect to obtain a positive resultwith the Diastix strip? Explain.
d) Imagine a student conducted the reaction in test tube #1 at a temperature of5.0 °C instead of 37 °C. Would the glucose concentration be increased or decreased from 500 mg/dL ? Explain.
e) Imagine a student conducted the reaction in test tube #1 at a temperature of50 °C instead of 37 °C. Would the glucose concentration be increased or decreased from 500 mg/dL ? Explain.
True or False: Anaplerotic and amphibolicare different words for the same thing.
O TrueO False
Ketosis results primarily from:
Too little protein in the diet
Too little fat in the diet
Too little carbohydrate in the diet
Too many carbohydrates in the diet
The Krebs Cycle and beta-oxidation of fatty acids are similar in that:
Both involve oxidative decarboxylation of their substrate.
Both involve reduction of NAD* to NADH.
O Both involve dehydration followed by hydration of the substrate.
Both involve "sliding an -OH."
Fatty acid synthesis and beta-oxidation are similar in that:
The reactions for synthesis are the exact reverse of the reactions involved in beta-oxidation.
Both involve changing the length of the fatty acid chain by 2 carbons at a time.
Both involve NADP+/NADPH.
Both occur in the mitochondrial matrix.
In which of the following pathways does a non-oxidative decarboxylation reaction occur? (Select all that apply! This is a hard one,so think about how you tell whether or not a reaction is oxidative.)
O The PDH
The Krebs Cycle
Fatty Acid Synthesis
The Krebs Cycle and beta-oxidation of fatty acids differ in that:
The Krebs Cycle happens in mitochondria, while beta-oxidation is in the cytoplasm.
Acetyl-CoA is a reactant in the Krebs Cycle but a product of beta-oxidation.
Only the Krebs Cycle involves production of NADH.
Only the Krebs Cycle involves "growing a carbonyl."
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