Macmillan Learning
The absorbance at 470 nm of the Fe(II)-phenanthroline complex was measured for several different Fe²+ concentrations to
create a calibration curve. The absorbance at each Fe²+ concentration was measured five times. The absorbance values, already
corrected for the blank absorbance, are shown in the table.
[Fe²+] (µm)
75.9
53.5
37.7
15.2
182.3
45.575
m=
0.4152
A₁ A₂ A3 A₁ As
0.582 0.591 0.587 0.583 0.588
0.412 0.411 0.421 0.414 0.418
0.264 0.266 0.269 0.258 0.266
0.109 0.113 0.108 0.115 0.111 0.1112
1.367 1.381 1.385 1.370 1.383 1.377
0.2646
average:
0.3418 0.345 0.346 0.343 0.346 0.3443
Use the averages of the replicate absorbance measurements to determine the slope and y-intercept of the best-fit straight line.
b=
sum:
0.00793
0.177
Incorrect
average y, (, - y)²(x₁ - x)²
0.0585 919.6
0.5862
62.8
62.0
Attempt 2
0.0050
0.0063
0.0543
0.1242
922.6
1967
HM-1
Fig: 1