A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas
chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The .
results of a preliminary run, which used a solution known to contain 1.83 mg/mL of methyl
benzoate (peak A) and 2.01 mg/mL of butyl benzoate (peak B), are shown. The area of peak A
is determined to be 304 and the area of peak B is determined to be 3.60 x 10² measured in
arbitrary units by the computer.
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To measure the sample, 1.00 ml. of a standard sample of butyl benzoate containing 2.37 mg/ml.
is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of
469 for peak A and 411 for peak B.
What is the concentration of methyl benzoate in the plant stream?
methyl benzoate concentration:
Lic
Detector Response
10
Time (min)
15
mg/mL
Fig: 1