Results to use for your Gram Stain Data Sheet Use for #8 on your Gram Stain Data Sheet Use for #17 on your Gram Stain Data Sheet Note: The answers to question #1 must be written in the format shown on the photo; after the equal sign the answer is either pink, purple or colorless. Name Date Lab Section I was present and performed this exercise (initials) Gram Stain OBSERVATIONS AND INTERPRETATIONS 1 Record your observations in the table below. Use separate lines for different organisms found in the Gram stain of your gumiline. Include a drawing of your own epithelial cells. #8 #17 Organism or Source Cellular Morphology and Arrangement Cell include a detailed sketch of a few representative cells) Dimensions DATA SHEET 3-6 Color MCB2010Lab Gram Reaction 139 19 QUESTIONS (1 Predict the effect on Gram-positive and Gram-negative cells of the following "mistakes" made when performing a Gram stain. Consider each mistake independently. Pink, PURPLE or Colorless a. Failure to add the iodine. Gr + Gr b. Failure to apply the decolorizer. Gr + 65 c. Failure to apply the safranin. 6r+ 6r. d. Reversal of crystal violet and safranin stains. 6r+ 2)Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they end up staining Gram-positive and Gram-negative cells differently in the Gram stain. 3 If you saw large, eukaryotic cells in the preparation made from your gumline, they were most likely your own epithelial cells. Are you Gram-positive or Gram-negative? (You can make a good guess about this even if you didn't see your cells.) 4 One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negati control organisms on her Gram stain of an unknown species. Her choices of controls were Escherichia coli an Bacillus subtilis. She tries several times and each time concludes she is decolorizing too long because both Results to use for your Acid Fast Data Sheet 1 Use for Mycobacteria on your Acid Fast Data Sheet Use for Enterococcus on your Acid Fast Data Sheet pro Namo Date Lab Section I was present and performed this exercise (initials) 1 Record your observations in the table below. Organism Enterococcus my bactria Acid-Fast Stains OBSERVATIONS AND INTERPRETATIONS Staining Cellular Morphology and Arrangement Method (include a detailed sketch PROK of a few representative cells) к FERIE K Cell Dimensions Color DATA SHEET 3-7 Acid-Fast Reaction (+/-)