stion 3 of 5 greater than the data in the table are used to create a c
stion 3 of 5 >
The data in the table are used to create a calibration curve for the determination of RNA from
its absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
Absorbance at 260
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
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