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The data in the table are used to create a calibration curve for the determination of RNA from

its absorbance at 260 nm.

Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the

best straight line fit to the calibration curve. Do not subtract the blank reading when creating

the calibration curve.

m=

b=

RNA

(µg)

0.00

10.36

20.72

31.08

41.44

Absorbance at 260

nm

0.268

0.497

0.795

1.131

1.373

Hg-1

Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of

0.699.