1st submission Student needs 2 Jobs and 2 Business (companies names) Based on instructions of previous work topic Poster Title New strategies to produce pseudotyped viruses to study entry of Flaviviruses

Synopsis A pseudotyped virus (PV) is a viral particle harbouring the core structure of one virus and the envelope (Env), the viral protein which interacts with the cellular receptor mediating entry, of a heterologous virus: these PV have been largely employed in gene therapy, serology and virus-host interaction studies. The use of PV is particularly important to study viruses classified as hazard group 3 and 4, as they can be handled at a lower containment level (BSL1/2) and do not require enhanced security, such examples are Ebola and other filoviruses, Lassa fever, Coronaviruses. However, those viruses which assemble within the intracellular membrane systems are particularly difficult to pseudotype, as the incorporation of their Env on the surface of a PV is compromised by the endoplasmic reticulum (ER) retention signal present on their Env. These include viruses which have caused or have the potential to cause an outbreak such as flaviviruses, e.g. Zika virus, Dengue virus and Japanese Encephalitis virus (IEV). Please note: The poster title has been set and the title cannot be changed.