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Q1:Description You are required to write an essay on the industrial manufacture of L-amino acids (you may select one type as a case study). No words/format limit but require concise writing. 601080 Industrial Bioprocess and Chemistry in Industry Assessment II.pdfSee Answer
Q2: Research Methodology Spring 2023- 2024 Exercise on Planning Your Research Project Prepare an outline of a project proposal which includes the following four sections. Your answers should be fairly short (a paragraph or two), with the total length of the assignment no longer than 3- 4 pages. We are looking for quality of thought, not quantity. Submit your assignment by e-mail. 1. Introduction: Introduce your research project using some related scientific articles and statistics. Cite the relevant references (use APA format). 2. Problem Definition (Justification) Describe the problem you plan to address and state why the intended research is important. This problem should not be too broad, but should focus on an important research question which can be addressed in your Master's Program. Cite the relevant references (use APA format). 3. Research Objectives List the objectives of the proposed research stating a one sentence general objective (or goal) and a numbered list of specific objectives. 4. Methods and Procedures Describe how the research will be conducted. Generally, what kind data will you collect and what analytical techniques or procedures do you expect to use? 5. Expected outcomes Briefly describe the expected outcomes of this proposed project. 6. Resources Needed Briefly consider the kinds of resources you will need to conduct the research (time, money, information ...) after considering the resources needed, you may need to make some adjustments in the above sections to be realistic. 7. References Format your references using APA styleSee Answer
Q3:Description You are required to write an essay on the industrial manufacture of L-amino acids (you may select one type as a case study). No words/format limit but require concise writing. 601080 Industrial Bioprocess and Chemistry in Industry Assessment II.pdfSee Answer
Q4: Standard constants: Take quiz Exit = 1 tonne 1,000 kg Atomic masses: C = 12, H = 1, O=16, N = 14, S 32 T(°c) =T(K) - 273 R = universal gas constant = 8.314 J mol-1 K-1 = == 1.987 cal mol 1 K-1 Question 1 2 pts The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH 7.0 and 20 °C to produce Glutamic acid for the manufacture of Mono Sodium Glutamate, a flavour compound for the food industry. The time course data for a typical batch culture of this bacterium in a fermenter vessel are given below: Take quiz typical batch culture of this bacterium in at fermenter vessel are given below: Cell Time Glucose Glutamic acic (h) concentration concentration concentration (g 1-1) (g 1-1) (g 1-1) 1.222 100 0 5 1.492 98.0 2.5 10 1.649 97.0 7.5 15 2.719 90.4 20.0 20 4.482 76.9 34.0 25 7.390 48.1 43.0 30 12.183 20.6 47.5 35 20.086 3.4 48.0 40 21.250 1.1 48.3 45 21.260 0.1 48.5 Exit Take quiz Exit 35 20.086 3.4 48.0 40 21.250 1.1 48.3 45 21.260 0.1 48.5 Based on the above data, determine the maximum specific growth rate of Corynebacterium glutamicum. O 0.1 min 1 O 0.063 h-1 0.0034 min 1 O1s 1 O 0.08 g 1-1 s-1 0.003 h-1 O 0.1 h-1 O 0.74 h-1 21.2 s 1 none of the above Question 2 Take quiz Exit ...continuation from question above... 2 pts The fermenter vessel is to be harvested for the glutamic acid in the liquid phase, once the carbon substrate (glucose) is completely consumed. How many production scale fermenters, each of 150 m³ operating liquid volume, would be needed to make 10,000 tonnes per year of Glutamic acid? Each batch. vessel is operated for the harvest time of complete carbon substrate utilisation (45 hours), and takes an additional 3 hours to load with liquid medium and inoculate with bacterial culture, and 2 hours to drain and sterilise after the aerobic batch fermentation. Take a process year to be 325 days. (Write in the box below the number of fermenter vessels needed. Do not enter any text, only a number)See Answer
Q5: Assignment description: A standard monoclonal antibody (mAb) manufacturing process was considered in this work, as shown in the Figure below. Fermentation Protein A Capture Anion Exchange Cation Exchange Depth Centrifugation Filtration VI I- UF/DF UF/DF VRF UF/DF Upstream Process Downstream Process The volume of bioreactor broth generated during each batch was 2500 L with a titre of 2g/L. Biomass and other debris were removed using centrifugation and depth filtration with step recovery yields of 95%. The mAb downstream processing sequence was defined as Protein A affinity chromatography capture step, low pH virus inactivation, ultrafiltration/diafiltration (UFDF), anion exchange chromatography (AEX), ultrafiltration (UF), cation exchange chromatography (CEX), virus reduction filtration (VRF) and a final UFDF. In order to mimic batch-to-batch variability caused by fluctuations in real manufacturing conditions, Monte Carlo simulations have been introduced to simulate the manufacturing process under uncertainty. Tasks: 1. Use descriptive and diagnostic analysis to identify the bottleneck (mass loss) of biomanufacturing downstream process. 2. Apply different supervised learning methods (decision tree and neural network) to predict the process mass loss from process parameters. 3. Provide insights about the discovery from the machine learning models. 4. Choose appropriate evaluation methods to compare different models. Data description: The spreadsheet, Bioprocess.xlsx, is a raw dataset collected directly from simulation software. It contains two data sheets: Mass loss and Key process parameters. "Mass loss” worksheet contains the mass loss information for each step: StepID, a-h, refers to the unit operation in the downstream processing sequence, from Protein A affinity chromatography to final UFDF; accordingly, Run number, 1-1000, refers to the Monte Carlo simulations runs. The "Key process parameter" worksheet contains the process fluctuation in key process parameters of each step during 1000 Monte Carlo simulation runs. The letter Y refers to step yield and EV refers to elution volume for the chromatography step. The numbers 1-8 refer to the sequence number in downstream processing, from Protein A affinity chromatography to final UFDF accordingly. For each UFDF step, there are three key parameters related to the flux rate which are average flux rate (AvgFlux), permeate flux rate (PermFlux) and overconcentration flux rate (OverFlux).See Answer

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