5 p53 is activated under cellular stress and turns on genes involved i
5. p53 is activated under cellular stress, and turns on genes involved in stress response. In the absence of
stress its levels are kept low by proteasomal degradation through interactions with MDM2 ubiquitin ligase that
marks it for degradation. (17 pts)
A. Identify at least three hydrogen bonding interactions that stabilize the p53-MDM2 complex? (3 pts)/nB. Use the secondary structure prediction web server Proteus (http://www.proteus2.ca/proteus2/) and
underline the residues that will participate in helix formation (2 pts):
C. Based on Borcherds et al, which residue in the peptide can be mutated to increase the helical structure
of the peptide? Is this in line with the prediction from (2) above (2 pts)?
D. How does the Kd between p53 and MDM2 change upon mutating Pro27 to Ala (3 pts)?/nE. What assay was used by Boercherd's et al to determine the effect of Pro27Ala mutation on DNA
replication post DNA damage (3 pts)?
F. What is the effect of Pro27Ala mutant on cell cycle progression post DNA damage? Based on this result
what is the mechanism by which p53 regulates cell fate after DNA damage? (4 pts)
*The amount will be in form of wallet points that you can redeem to pay upto 10% of the price for any assignment. **Use of solution provided by us for unfair practice like cheating will result in action from our end which may include permanent termination of the defaulter’s account.Disclaimer:The website contains certain images which are not owned by the company/ website. Such images are used for indicative purposes only and is a third-party content. All credits go to its rightful owner including its copyright owner. It is also clarified that the use of any photograph on the website including the use of any photograph of any educational institute/ university is not intended to suggest any association, relationship, or sponsorship whatsoever between the company and the said educational institute/ university. Any such use is for representative purposes only and all intellectual property rights belong to the respective owners.