Q8. 5E83 - (10 points total; 2 points each) A. Based on this PDB, does the compound GBT440 stabilize the S or the R state?/nB. Draw an arrow pushing mechanism for the reaction between GBT440 and the alpha subunit residue amine group. % O₂ Saturation 100 80 60 40 20 0 p50 (mmHg) -30 μmol/l -10 μmol/l -6 μmol/l -0 μmol/l 20 40 15 10 -6 -5 GBT440 Log [M] 60 pO₂ (mmHg) 80 100 120/nC. Based on this experiment, what state does GBT440 stabilize? D. Why is more GBT440 needed in the blood compared to purified protein? 100 80 60 %0₂ Saturation 20 0 0 20 (6Hww) od -1000 μmol/l - 600 μmol/l - 300 μmol/l - 0 μmol/l 40 60 20 10 45-40 -35 -30 -25 GBT440 [Log M 80 pO₂ (mm Hg) 100 120/nE. What could be a side effect of GBT440? Will you want this person to do much exercise when taking this drug? Why?

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