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Lab 2 Writing Assignment Due: Thu Apr 18, 2024 11:59pm Attempt 1 In Progress NEXT UP: Submit Assignment 10 Points Possible Add Commen Unlimited Attempts Allowed ✓ Details Please refer to this Google document describing the Lab 2 "Lab Writing Assignment #1" for instructions. Once finished with your assignment, submit it here to Canvas as a Word document or pdf that includes 1) Your captioned image (= figure (image) + caption) 2) Your compare and contrast (table & paragraph) 3) Your scored grading rubric table Please ask if you have any questions! ✓ View Rubric Ⓡ Σ B hp 31 Submit Assignment Ne Apr 18 2:44/n/n ** This document is being shared as a viewable document only because this document is being used by everyone taking Biology 211 this quarter. Should you wish to answer questions or take notes about this lab, please copy and paste this document into another file and annotate the document there. ** BIOL& 211 | Lab 2 | Compound Microscope and Cellular Diversity Credit: CCO Public Domain Part 1: Concepts of Microscopy Objectives • Learn how to use microscope “field of view” dimensions to estimate the size of objects viewed with the microscope. Understand some essential microscope terminology, including compound (or light) microscope, field of view, depth of field, magnification, contrast, and resolution. INTRODUCTION Humans are visual creatures, obtaining much of our information about the world around us by using our eyes. Not surprisingly, some of the most useful tools in biology are those that allow us to visualize objects, processes, and phenomena. Powerful computers and other recent technologies today allow us to visualize and explore as never before. But even these cannot replace one of the most fundamental tools of biology, the microscope. With the microscope we can see things that are too small for the naked eye. There are many different kinds of microscopes (see your textbook). The one you will familiarize yourself with today is the compound light microscope. In this online lab activity we will explore how to use and care for this most basic of biological tools that has unlocked so many mysteries of the biological world! PRELAB As preparation for the lab: 1. Watch this video on how to use a microscope: https://www.youtube.com/watch?v=xzjowD1KN20 2. Familiarize yourself with the following terms in microscopy: Field of view: Refers to the area you see when you look through the microscope. As you observe a specimen using the three different objective lenses, note how the field of view changes with magnification. • Depth of field: Refers to the vertical distance (thickness or depth) that is in focus at • the same time. The depth of field also changes with magnification. Magnification: The apparent increase in size • Contrast: Refers to the degree of difference between dark and light areas of the • specimen. High contrast is helpful in bringing out details. Resolution: The ability to see two closely spaced objects as separate ** As you go through the cell size estimation activity in the next couple of pages, think about how the above terms are important in generating the images that you see. 1 Activity: Cell Size Estimation In this activity, you'll learn how to use scale bars to calculate the size of specimens visualized with a compound microscope. To start, please watch the following video: https://www.youtube.com/watch?v=E_NiyAt7pRM 1. Determining the size of a red blood cell Observe the image of red blood cells. 50μm Notice that the scale bar says "50µm,” which is 50 micrometers or 0.05 mm. First, estimate how many blood cells could fit end to end along the scale bar. Now, use that estimate and the equation below to calculate the size of an individual blood cell: 0.05 mm (length of scale bar) Number of cells that fit along scale bar Your calculations: 2 _mm (diameter of 1 cell) 2. Determining the size of a Volvox colony Observe the image of Volvox globator. 150μm Notice that the scale bar says “150 µm,” which is 150 micrometers or 0.15 mm. Estimate how many of the Volvox can fit along the scale bar. Does one entire Volvox fit, or does only part of one fit on the scale bar? Show your work below. Your calculations (include the correct equation for this problem, as modeled in the first problem): 3 3. Determining the diameter of a sea urchin sperm Observe the image of sea urchin sperm. 50μm Notice that the scale bar is 50 μm. Determine the diameter of the head (round portion) of a sea urchin sperm. Your calculations: 4/n INSTRUCTIONS FILE Here you have to make the both lab report as they are dependent In case you have to choose anything you will 1 choose all bacteria and everything required by yourselves

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