Assume the mating of two dogs from different breeds that both show an excessive bleeding disorder. In one breed, the condition is an autosomal recessive, and in the other a sex-linked recessive. The dogs used are an affected male Lab and an affected female Doberman. The progeny resulting from the mating are: 1. All normal females 2. All affected males Which breed has the sex-linked disorder and what is the genotype for that breed at both loci?

Based on the following strand of mRNA, what was the original template strand of DNA? 5'-AUCGCGCCA-3¹ 5'-ATCGCGCCA - 3¹ 3'-ATCGCGCCA-5' 3'-TAGCGCGGT - 5' 5'-TAGCGCGGT-3'

Which of the following is true of RNA primers? They are only used in building on the lagging strand There are numerous RNA primers used when building on the leading strand Once replaced, they are known as Okazaki fragments They create attachment points for the DNA polymerase to attach to and begin laying down nucleotides in a 5'--> 3' direction

Suppose an individual had a mutation in their gene that codes for the production of DNA polymerase I. What issues would this individual experience? Explain by referring to specific concepts learned in class.

17. Use the DNA sequence 5'-ATCTCCTGGTAA-3' to demonstrate the effect of each type of mutation on the resulting polypeptide sequence. Include sequences for DNA, mRNA, and polypeptide. a) Missense mutation b) Frameshift mutation Considering the following DNA sequence: 3' CCGTACCCTGTATAAAAC - 5' a. Write the complementary DNA strand that would be produced in replication (A/1): b. Write the complementary mRNA strand that would be produced in transcription (A/1): c. What is the sequence of amino acids that would result from translation of the mRNA sequence? (A/1)

While studying for your molecular genetics test, your friend says that during translation, the polypeptide chain is always located in the P site of the ribosome. Is your friend correct? Refer to the process of polypeptide production in the ribosomes to explain.

Which of the following is true of mRNA? It is oriented in a 3'- 5' direction It is complimentary to the coding strand of DNA It is identical to the coding strand of DNA None of the above are true

Which of the following is not true when comparing DNA and RNA? DNA uses thymine, RNA uses uracil DNA has a deoxyribose sugar. RNA has a ribose sugar DNA has a 3' H, whereas RNA has a 3'OH All of the above are true

Which of the following enzymes is responsible for creating phosphodiester bonds between Okazaki fragments and recently replaced primers? DNA polymerase 1 DNA polymerase II RNA polymerase DNA ligase

Lab Report 2: PCR and qPCR REMINDER: We do not want either a repeat of the Lab Manual or a repeat of everything you recorded in your Lab book. (Marks will be deducted for failing to adhere to this.) 1. Briefly describe the steps involved in a Polymerase Chain Reaction. 2. Attach a properly labelled photograph of amplicons from PCR. Estimate the sizes of any "bands" or size range of any "smears" that can be discerned from the Ladder. 3. Present a table of your NanoDrop quantification of "purified PCR products". Comment on the yield and the purity of samples. Be sure to address any anomalies. 4. Briefly describe the "Types" of qPCR used to quantify gene expression. Comment on the type of qPCR you have used to quantify the expression of your gene of interest. 5. Provide a table of your qPCR data analysis and present the results in a bar diagram with proper labeling. 6. Summarize the results from your qPCR experiment. Comment on whether these results reinforce or contradict your initial hypothesis on the expression of your gene of interest in Ras-transformed cells compared to the control cells? Additional Resources if you need them: • The sizes of the DNA fragments in the DNA Ladders are in Appendix #2.